Measuring an off-target effect in a compound that was meant to be a specific receptor tyrosine kinase inhibitor. Hela cells were incubated with either a vehicle (left image) or the drug for 72 hours (right image). The cell nuclei were then stained with Hoechst 33342 (blue channel), and the cytoskeletal components actin and tublin with Phalloidin 555 (green channel) and anti-tublin (red channel) respectively. It is clear from this example that the RTK inhibitor is causing a major rearrangement of the cytoskeleton and so is not specific to the desired target.

We have a very diverse set of assays measuring many aspects of toxicity. Whether it is a single measure of cell viability or a more complex set of assays aimed at predicting in vivo toxicology, we have the tools to meet your requirements.

Academics can greatly speed up their research by switching from Western blot techniques to our in-house assays in a 96 well plate format.

As well as basic research, industry will find our assays highly useful in predicting toxicity of their compounds in animal models. This allows elimination of the compound series at an earlier point in the drug discovery pipeline and thus is very cost effective.

Toxicity assays include:

• Apoptosis/cell cycle
• DNA damage
• Genotoxicity
• Oxidative stress
• Phospholipidosis
• Superoxide production