Our apoptosis assay is a beautiful example of the utility of High Content Biology in detecting subtle protein changes which are completely missed using Western analysis. The pro-apoptotic protein BAK undergoes a conformational change which results in the uncovering of its N-terminus. This event can be measured by fluorescence microscopy using an antibody to the N-terminus of the BAK protein. However, normal Western analysis completely fails to detect these changes because the Bak protein is fully denatured by the sample preparation buffers and so all conformational information about the protein is lost.

Full High Content Screening Assay List

At Imagen Biotech, we have a unique solution to this problem in that we rapidly create complex Excel Workbooks which contain all the raw data from an experiment in its original plate format along with the same data associated with all the independent variables of the experiment.

This allows us to offer our customers a truly flexible service where the customer controls all aspects of plate layout from the number of doses used to the number of replicates on a plate. We can even change the layout in different experiments to control for any positioning artefacts.

Push the buttons below to step through the pages of a small workbook we generated in house so you get an idea of how the data we generate will be returned to you. The data in the workbook is an example of a dual phospho and Total EGFR assay.